UGA-42 Firefly
High-precision, high-power density, small spot illumination system
- Scanner-based illumination system
- Spot size approaching the diffraction limit
- User-designed spatial & temporal illumination control
- Up to 4 fiber-coupled lasers ranging from near UV to near IR
- Available for many research microscope and macroscope models
- Easily integrated into imaging and electrophysiology rigs
- Wide variety of applications
Designed for dynamic illumination of points or selected regions in the field of view of the microscope, the UGA-42 Firefly adds a variety of photomanipulation techniques to imaging and electrophysiology rigs. It supports up to 4 fiber-coupled lasers ranging from near UV to near IR, allowing multiple methods to be used on the same microscope.
The software runs in parallel with and independent of the imaging software for uninterrupted imaging during photomanipulation. The photomanipulation is done either in real-time at the click of the mouse or by setting up sequences of regions of interest (ROIs) illuminated during the experiment with accurate timing. Setting up the ROI sequence is facilitated by the use of a timeline for temporal control and user-friendly editing functions.
| Laser Coupling | Optical fiber |
| Laser Type | Diode lasers |
| Spot Size | Submicron spots can be obtained on most microscope models using single-mode fibers and high NA objectives |
| Switching Time | < 1 ms between any locations in the field of view |
| Maximum Number of Lasers per System | 4 |
| Maximum Number of Lasers per Experiment | 4 |
| Switching Time Between Lasers | Microsecond range |
| ROI Geometries | Circles, straight lines, rectangles, and user defined polygons & lines |
| Galvanometer Calibration | Autocalibration algorithm |
| Synchronization with Other Devices | Bidirectional TTL communication (slave or master) with other devices |
| Laser Modulation | Analog and digital |
| Built-In Safety Shutter | Yes |
| Built-In Interlock | Yes |
- Add-on optical module for research microscopes
- Simultaneous and independent spot illumination and imaging
- Quiet operation for integration into electrophysiology rigs
- Spot size as low as submicron, depending on the microscope model, objective, and fiber diameter
- The laser spot can be located anywhere in the field of view of the microscope or scanned over user-designed regions of interest
- ROI geometries include standard shapes (straight lines, circles, rectangles) or user-designed polygons and lines
- Versatile and user-friendly editing functions for spatial and temporal objects
- Autocalibration algorithm
- Submillisecond switching time between any two positions in the field
- Up to 4 fiber-coupled lasers per system; all lasers can be used within the same experiment
- Rapid switching between different lasers
- All lasers feature analog and digital modulation and built-in safety shutter and interlock
- “Click-and-Fire” mode for real-time localized illumination in points or user-designed ROIs
- “Sequence mode” programmable illumination with user-designed spatial and temporal control
- Two TTL inputs and two TTL outputs for synchronization as slave or master with other devices
- Manual or TTL sequence start
- Synchronization of individual sequence ROIs with other events in the experiment
- Runs in parallel with and independent of any imaging software
- Communication protocols available for Metamorph, Zeiss ZEN2, ZENBlue, ZENBlack, Nikon Elements, Micromanager
- Can be combined with UGA-42 Geo and UGA-42 Caliburn modules to create multifunctional setups
- Uncaging/Photolysis
- Optogenetics
- Neural mapping
- Photoconversion/Photoswitching
- FRAP/Photobleaching
- FRET
- Selective, localized illumination of photosensitive materials
- Localized heating and temperature-jump
Downloadable Documents
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