UGA-42 Caliburn
High-precision, modular, high-power density, ablation system
- Scanner-based system for controlled damage at subcellular level
- Spot size approaching the diffraction limit
- High-power density
- User-designed spatial & temporal illumination control
- Directly coupled pulsed lasers (various peak power levels at 266nm, 355 nm, 532 nm)
- Available for a large number of current research microscopes
- Easily integrated into imaging rigs
The UGA-42 Caliburn is a galvanometer based localized illumination system for laser induced damage in cells, tissue, and organisms. The damage is done under highly controlled parameters in points or selected regions in the field of view of the microscope.
The UGA-42 Caliburn uses pulsed UV lasers and features built-in motorized laser focus, safety shutter, interlock, and digital laser modulation. A built-in attenuator ensures fine power adjustment.
The photomanipulation and control software runs independently, allowing uninterrupted imaging during photomanipulation.
For moving or changing samples, the ablation can be done in real-time (“Click & Fire” mode), with predetermined temporal parameters, at locations chosen by the user during the experiment. For fixed samples, in addition to the “Click & Fire” option, sequences of ROIs with user-designed spatial and temporal parameters can be set up prior to the experiment (“Sequence” mode) and run at the click of a button or synchronized with other events via TTL pulses.
The Caliburn is a versatile add-on to most imaging (confocal, spinning disk, wide-field, etc.) microscopes.
| Laser Coupling | Via optics |
| Laser Type | Pulsed, multiple options of pulse energy available |
| Repetition Rate | 1 kHz |
| Spot Size | Submicron spots can be obtained on most microscope models using high NA objectives |
| Switching Time | < 1 ms between any locations in the field of view |
| Maximum Number of Ablation Lasers per System | 2 |
| Maximum Number of Lasers per System | 4 |
| Maximum Number of Lasers per Experiment | 4 |
| Switching Time Between Lasers | Microsecond range |
| ROI Geometries | Circles, straight lines, rectangles, and user-defined polygons & lines |
| Galvanometer Calibration | Autocalibration algorithm |
| Synchronization with Other Devices | Bidirectional TTL communication (slave or master) with other devices |
| Laser Modulation | Digital |
| Built-In Shutter | Yes |
| Built-In Attenuator | Yes |
- Add-on optical module, can be integrated into most of the current models of research microscopes
- Simultaneous and independent ablation and imaging
- Spot size as low as submicron with high NA objectives
- The laser spot can be located anywhere in the field of view of the microscope or scanned over user-designed regions of interest
- ROI geometries include standard shapes (straight lines, circles, rectangles) or user-designed polygons and lines
- Autocalibration algorithm
- Fast switching (<1 ms) between any two positions in the field
- “Click-and-Fire” mode for real time ablation in points or user-designed ROIs for moving/changing samples
- “Sequence mode” programmable illumination with user-designed spatial and temporal control for non-changing samples
- Versatile and user-friendly ROI and timeline editing functions
- Communication protocols with Metamorph, Zeiss ZEN2, ZENBlue and ZENBlack, Nikon Elements, Micromanager
- Compatible with any imaging software
- Digitally modulated pulsed lasers featuring motorized focus, safety shutter, and interlock
- Fine power control via built-in attenuator
- Two TTL inputs and two TTL outputs for synchronization as slave or master with other devices
- Manual or TTL sequence start
- Synchronization of individual ROIs with other events in the experiment
- Combinations with Firefly and Geo modules up to a total of four lasers. All lasers can be used within the same experiment with submillisecond switching times between lasers.
- Ablation
- Microdissection/nanosurgery
- DNA damage
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