OptoSplit III LS Image Splitter

Easy-to-align image splitter that enables a single camera to record images simultaneously at three different emission wavelengths

User-friendly design requires minimal maintenance and makes alignment simple
Excellent for use in FRET, TIRF, and spinning disk confocal applications
Compatible with all major brands of camera, including large sCMOS chips

The OptoSplit III LS has been designed as a convenient, inexpensive solution for simultaneous multi-wavelength imaging. Traditionally, multi-wavelength imaging experiments required that multiple images be captured sequentially. However, because the various images were not taken at the same time, temporal artifacts, caused by intracellular movement, often appeared between exposures. To eliminate these temporal artifacts and provide the most accurate measurements, the OptoSplit III allows three images to be acquired simultaneously.

The images captured by the OptoSplit III contain information from three different emission bands and can be processed using commercially available software packages. Alternatively, the images can be processed manually. While it is optimized for coupling to a scientific microscope, the OptoSplit III can also be used with camera lenses or any other system of lenses that produce an image plane of a suitable size.

Standard Spectral Range	450 nm – 900 nm
Dimensions	122 mm (L) x 374 mm (W) x 79 mm (H)
Weight	1.80 kg
Emission filter size	25 mm
Dichroic mirror size	25.5 mm x 36.0 mm or 26.0 mm x 38.0 mm2.0 mm thick, UltraFlat dichroics highly recommended

Supports large sensors up to 22 mm diagonal	Large field of view, including large sCMOS chips
User exchangeable dichroic mirrors	Easily reconfigure system for different experiments
Adjustable input aperture	Precise definition of the desired field of view
Supports dichroic mirrors up to 3 mm thick	Distortion-free images
Stable alignment	Long-term time-lapse imaging
Optional auxiliary filter holders	Add chromatic corrective lenses, supplemental emission filters, neutral density filters, or lens for multiplane microscopy
Accepts 2- or 3-channel emission splitting cubes	Allows the OptoSplit III to seamlessly move between 2-channel and 3-channel imaging

Ratiometric, calcium, voltage, and pH imaging
Triple probe widefield imaging
Förster Resonance Energy Transfer (FRET)
Total Internal Reflection Fluorescence (TIRF)
Spinning disk confocal microscopy
Polarisation/Anisotropy
Multifocal Plane Microscopy (MUM)
Combined fluorescence/transmitted light microscopy

Downloadable Documents

Please find links below to relevant downloads for our products.

MultiCam LS Image Splitter

Highly flexible image splitter that enables multi-camera, simultaneous multi-channel imaging from a single microscope port

Supports up to 4 channels of varying polarity, emission wavelength, and depth of focus
Essential equipment for researchers wanting to push the limits of simultaneous multi-channel imaging

(0 reviews)

Learn more