OptoSplit II LS Image Splitter
Easy-to-align image splitter that enables a single camera to record images simultaneously at two different emission wavelengths
- User-friendly design requires minimal maintenance and makes alignment simple
- Excellent for use in FRET, TIRF, and spinning disk confocal applications
- Compatible with all major brands of camera, including large sCMOS chips
The OptoSplit II LS has been designed as a convenient, inexpensive solution for simultaneous dual wavelength imaging. Traditionally, dual-wavelength imaging experiments required that two images be captured sequentially. However, because the two images were not taken at the same time, temporal artifacts, caused by intracellular movement, often appeared between exposures. To eliminate these temporal artifacts and provide the most accurate measurements, the OptoSplit II allows both images to be acquired simultaneously.
The images captured by the OptoSplit II contain information from two different emission bands, and can be processed using commercially available software packages. Alternatively, the images can be processed manually. While it is optimized for coupling to a scientific microscope, the OptoSplit II can also be used with camera lenses or any other system of lenses that produce an image plane of suitable size.
| Standard Spectral Range | 450 nm – 900 nm |
| Dimensions | 110 mm (L) x 261 mm (W) x 79 mm (H) |
| Weight | 1.26 kg |
| Emission Filter Size | 25 mm |
| Dichroic Mirror Size | 25.5 mm x 36.0 mm or 26.0 mm x 38.0 mm2.0 mm thick UltraFlat dichroics highly recommended |
| Camera Size Supported | Up to 22 mm diagonal |
| Supports large sensors up to 22 mm diagonal | Large field of view, including sCMOS detectors |
| User exchangeable dichroic mirrors | Easily reconfigure system for different experiments |
| Adjustable input aperture | Precise definition of the desired field of view; also supports cropped sensor mode found in many EMCCD cameras |
| Supports dichroic mirrors up to 3 mm thick | Distortion-free images |
| Stable alignment | Long-term time-lapse imaging |
| Optional auxiliary filter holders | Accommodates ND filters, chromatic correction lenses, supplementary emission filters, or lens for multiplane microscopy |
- Ratiometric, calcium, voltage, and pH imaging
- Dual probe widefield imaging
- Förster Resonance Energy Transfer (FRET)
- Total Internal Reflection Fluorescence (TIRF)
- Spinning disk confocal microscopy
- Polarization/Anisotropy
- Combined fluorescence/transmitted light microscopy
- Multifocal plane microscopy
Downloadable Documents
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