With traditional light sources, the long-term drift in light intensity may negatively impact quantitative imaging experiments. The OptoLED by Cairn Research solves this problem with ultra-high stability, digital vibration-free switching, and rapid intensity modulation. The ability to “gate” the light source directly from the camera and thus only expose the specimen while the camera is integrating reduces photobleaching and also prevents bleedthrough during frame readout.
The OptoLED controller is capable of driving two LEDs independently, over a wide range of currents. For pulsed illumination, switching times of less than 100 nanoseconds are achievable. Digital inputs are provided to switch each LED on and off independently, and sockets are provided to allow additional synchronization with the camera to reduce phototoxicity or motion artifacts. Optical feedback is included to prevent changes in light output that would otherwise result from variation in LED temperature.