L-SPI Single Plane Illuminator
Macro light-sheet illuminator for 3D imaging of large samples
- Live-cell imaging with ultra-low phototoxicity
- Spatial and temporal fractionation of illumination
- Create up to 4 orthogonal light-sheets to reduce shadowing
- Long travel piezo stage for precise z-steps
The L-SPI is designed to work with any macro- or microscope, single-mode fiber laser source, and scientific camera. This simple and effective instrument recombines two uniform, wide light sheets at right angles, reducing shadowing without the need for sample rotation or image fusion. The system includes optional sample chambers, fast long-travel piezo stage, and an optional second L-SPI head to bring sheets in from four directions. The L-SPI can be adapted to a wide range of experimental purposes. Its compact size and large freedom of access (e.g. for positioning micro-electrodes) make it a highly flexible solution.
| Number of laser lines | 2 or 4 orthogonal sheets possible |
| Sheet Thickness | 22 um with ~ 20 mm^2 imaging area 11 um with ~ 10 mm^2 imaging area |
| Field of View | 16 mm x 16 mm with 22 um thick sheet |
| Z Step Size | 10 nm minimum |
| Z Travel Range | 20 mm |
| Scan Speed | 10 mm/second |
| Generate 2 or 4 Orthogonal Sheets | Minimizes shading and scatter of illumination light in thicker samples |
| 22 um sheet thickness | Excellent axial resolution on macro samples |
| 16 mm x 16 mm Field of View |
Image larger samples in single field |
| 20 mm z-travel range |
Image large samples easily |
- Imaging of large, cleared organs and tissue sections
- Fluorescence imaging of photosensitive marine organisms, such as coral
- Fluorescence imaging of whole organisms, such as zebrafish
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